9UUF

Crystal structure of nanobody Tnb438 with MERS-CoV RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	192.664, 44.476, 116.734
Unit cell angles	90.00, 122.75, 90.00

Refinement procedure

Resolution	49.090 - 4.370
R-factor	0.3049
Rwork	0.304
R-free	0.32290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.941
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.090	4.528
High resolution limit [Å]	4.370	4.370
Number of reflections	5575	468
<I/σ(I)>	5.8
Completeness [%]	98.2
Redundancy	3.8
CC(1/2)	0.902	0.656

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M Na HEPES pH7.5, 2.0 M ammonium formate