9UUB
8xMT mutant of stilbene synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 104.640, 137.390, 178.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.150 - 1.410 |
| Rwork | 0.158 |
| R-free | 0.16910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.108 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.150 | 1.510 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Number of reflections | 244738 | 39234 |
| <I/σ(I)> | 18.01 | 1.15 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 13.47 | 13.62 |
| CC(1/2) | 1.000 | 0.478 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.25 M sodium citrate tribasic, 0.1 M HEPES pH 8.0 |
