9UUA
Stilbene synthase of Cenchrus americanus in complex with coenzyme A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 104.864, 137.304, 179.173 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.500 - 1.435 |
| Rwork | 0.175 |
| R-free | 0.19920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.867 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.500 | 1.510 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Number of reflections | 218886 | 30241 |
| <I/σ(I)> | 6.4 | 0.8 |
| Completeness [%] | 93.4 | 89.1 |
| Redundancy | 9.8 | |
| CC(1/2) | 0.965 | 0.374 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 10 %(v/v) Glycerol, 0.9 M Sodium Citrate Tribasic pH 7.0 |
