9UU9
Stilbene synthase of Cenchrus americanus in complex with resveratrol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 105.345, 137.422, 179.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.712 - 1.400 |
| Rwork | 0.161 |
| R-free | 0.17910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.177 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.712 | 1.380 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Number of reflections | 311348 | 45253 |
| <I/σ(I)> | 11.8 | 1 |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 13.1 | 11.2 |
| CC(1/2) | 0.997 | 0.434 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 5 mM resveratrol, 10 %(v/v) PEG 400, 10 %(v/v) Glycerol, 0.7 M sodium citrate tribasic pH 7.0 |
