9UU8

Stilbene synthase of Cenchrus americanus in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	104.915, 138.194, 179.791
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	109.567 - 1.410
Rwork	0.177
R-free	0.18990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.067
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.570	1.480
High resolution limit [Å]	1.410	1.410
Number of reflections	250737	36186
<I/σ(I)>	9.9	1.1
Completeness [%]	99.7	99.3
Redundancy	13.4	13.4
CC(1/2)	0.997	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10% Glycerol, 0.9 M sodium citrate tribasic pH 7.0