9UU7

The crystal structure of human DEAD-box RNA helicase DDX28 RecA1 domain in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97847
Spacegroup name	P 21 21 21
Unit cell lengths	43.260, 67.201, 147.501
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.730 - 2.600
R-factor	0.2313
Rwork	0.229
R-free	0.27710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.645
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.730	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.081	0.543
Rmeas	0.086	0.573
Rpim	0.028	0.180
Total number of observations	122515	15459
Number of reflections	13808	1646
<I/σ(I)>	16.1	4.2
Completeness [%]	99.6
Redundancy	8.9	9.4
CC(1/2)	0.998	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	25% PEG 3350, 0.2 M Lithium sulphat, 0.1M Tris, pH 8.5