9UU0

The structure of Bacteroides fragilis T6SS effector BteO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-20
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	234.120, 63.460, 113.610
Unit cell angles	90.00, 110.79, 90.00

Refinement procedure

Resolution	56.260 - 2.720
R-factor	0.2759
Rwork	0.275
R-free	0.29660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.589
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.260	2.817
High resolution limit [Å]	2.720	2.720
Rmeas	0.246	0.246
Rpim	0.093
Number of reflections	42024	42024
<I/σ(I)>	7.7
Completeness [%]	99.4
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	300	0.1M sodium citrate pH 3.9, 12% w/v PEG35000