9US9

Whole structure of WT human prostamide/prostaglandin F synthase(PGFS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97933
Spacegroup name	P 32 2 1
Unit cell lengths	116.605, 116.605, 77.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.170 - 3.380
R-factor	0.2486
Rwork	0.247
R-free	0.28040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.445
Data reduction software	xia2
Data scaling software	HKL-2000
Phasing software	MrBUMP
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.170	3.500
High resolution limit [Å]	3.380	3.380
Number of reflections	8498	827
<I/σ(I)>	8.2
Completeness [%]	96.3
Redundancy	5.5
CC(1/2)	0.990	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	10% (w/v) polyethylene glycol 20000 (PEG 20000)