9UPZ

KCMF1 Zn-coordinating domains with RTGG peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.282590
Spacegroup name	P 43 3 2
Unit cell lengths	97.347, 97.347, 97.347
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.420 - 1.710
R-factor	0.2094
Rwork	0.206
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9jni
RMSD bond length	0.011
RMSD bond angle	1.170
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((1.20.1_4487))
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.770
High resolution limit [Å]	1.710	1.710
Rmerge	0.161	2.464
Rmeas	0.164	2.539
Rpim	0.029	0.603
Number of reflections	17490	1687
<I/σ(I)>	23.61	1.52
Completeness [%]	99.5	98.77
Redundancy	31.2	16.7
CC(1/2)	0.999	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.3 M Magnesium chloride hexahydrate, 0.3M Calcium chloride dihydrate, 12.5% MPD, 12.5% PEG1000, 12.5% PEG 3350