9UPH
Crystal structure of Imine Reductase Mutant(AHtanRedAm) from Actinoalloteichus hymeniacidonis in complex with NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.215, 104.936, 94.208 |
| Unit cell angles | 90.00, 94.49, 90.00 |
Refinement procedure
| Resolution | 33.010 - 2.800 |
| R-factor | 0.2066 |
| Rwork | 0.204 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.549 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.1_3469) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.010 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.150 | 2.120 |
| Number of reflections | 31699 | 16053 |
| <I/σ(I)> | 7.29 | |
| Completeness [%] | 99.7 | 99.81 |
| Redundancy | 4.8 | |
| CC(1/2) | 0.993 | 0.733 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.10% w/v n-Octyl-b-D-glucoside, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 22% w/v PEG 3,350 |
