9UPA

Improved thermostability of a GH10 xylanase based on its X-ray crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-07
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	53.350, 69.300, 91.080
Unit cell angles	90.00, 91.91, 90.00

Refinement procedure

Resolution	46.694 - 2.600
Rwork	0.242
R-free	0.29470
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.694	2.760
High resolution limit [Å]	2.600	2.600
Number of reflections	39554	6333
<I/σ(I)>	5.64
Completeness [%]	98.7
Redundancy	3.43
CC(1/2)	0.961	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	0.1MBis-TrispH 7.0,25%(W/V) MPD