9UP9
Cystal Structure of Shark VNAR Nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-02-26 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.704, 64.704, 57.598 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.220 - 2.000 |
| R-factor | 0.2081 |
| Rwork | 0.207 |
| R-free | 0.23561 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8hgi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.741 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.223 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.173 | |
| Number of reflections | 8724 | 617 |
| <I/σ(I)> | 13 | |
| Completeness [%] | 99.9 | |
| Redundancy | 17.7 | |
| CC(1/2) | 0.993 | 0.826 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M CHES, PH 9.5, 20% W/V PEG 8000 |
