9UP7

anti-HPV18 E7 monoclonal antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-12
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	67.644, 54.698, 124.060
Unit cell angles	90.00, 102.11, 90.00

Refinement procedure

Resolution	50.260 - 2.100
R-factor	0.2124
Rwork	0.211
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.841
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.140	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.079	0.584
Rmeas	0.109	0.802
Rpim	0.074	0.548
Number of reflections	50750	7220
<I/σ(I)>	11.4	2.4
Completeness [%]	97.3
Redundancy	3.5
CC(1/2)	0.992	0.633

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	0.2M ammonium sulfate +26% PEG 4000