9UP3

Crystal structure of Ebola virus VP30 and its peptide NPL3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-24
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97915
Spacegroup name	P 1
Unit cell lengths	47.700, 53.726, 82.876
Unit cell angles	72.96, 81.28, 63.80

Refinement procedure

Resolution	46.600 - 2.040
Rwork	0.230
R-free	0.25730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.716
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.610	2.090
High resolution limit [Å]	2.040	2.040
Number of reflections	44381	3286
<I/σ(I)>	3.3
Completeness [%]	98.1	96.8
Redundancy	1.8
CC(1/2)	0.978	0.393

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M Cesium chloride, 15% PEG3350