9UOD
Crystal structure of pHBMT1 from Populus trichocarpa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 278 |
| Detector technology | PIXEL |
| Collection date | 2023-10-01 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.007, 53.153, 103.430 |
| Unit cell angles | 90.00, 105.51, 90.00 |
Refinement procedure
| Resolution | 99.660 - 1.820 |
| R-factor | 0.17926 |
| Rwork | 0.177 |
| R-free | 0.21573 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.804 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.660 | 2.200 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.131 | 0.175 |
| Rmeas | 0.152 | 0.204 |
| Rpim | 0.076 | 0.103 |
| Number of reflections | 45448 | 51489 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 95.1 | 99 |
| Redundancy | 4.1 | |
| CC(1/2) | 0.972 | 0.424 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | HEPES pH 7.6, Sodium sulfate decahydrate, PEG3350 |
