9UOC

Crystal structure of nanobody Tnb150 with MERS-CoV RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	80.926, 116.604, 87.058
Unit cell angles	90.00, 93.34, 90.00

Refinement procedure

Resolution	86.910 - 3.240
R-factor	0.2905
Rwork	0.287
R-free	0.33160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.629
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.910	3.360
High resolution limit [Å]	3.240	3.240
Number of reflections	24912	2157
<I/σ(I)>	7.2
Completeness [%]	96.9
Redundancy	5.8
CC(1/2)	0.954	0.311

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M Sodium malonate pH 4.0, 12%(w/v)PEG3350