9UO7

L. donovani Cell cycle associated protein MOB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978500
Spacegroup name	P 1 21 1
Unit cell lengths	33.381, 68.525, 46.449
Unit cell angles	90.00, 110.07, 90.00

Refinement procedure

Resolution	36.800 - 1.300
R-factor	0.1806
Rwork	0.179
R-free	0.19680
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.041
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.800	1.346
High resolution limit [Å]	1.300	1.300
Rmerge	0.040	1.395
Number of reflections	48179	4802
<I/σ(I)>	46.34
Completeness [%]	99.8
Redundancy	10
CC(1/2)	0.759	0.656

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	0.2M Ammonium acetate, 0.1M Sodium acetate trihydrate pH4.6, 30% PEG 4000