9UN4
Crystal structure of juvenile hormone acid methyltransferase castaneum JHAMT3 from Tribolium castaneum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.597, 71.476, 133.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.880 - 2.500 |
| Rwork | 0.245 |
| R-free | 0.29450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.523 |
| Data reduction software | HKL-3000 |
| Data scaling software | PROCOR |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.880 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.170 | 0.927 |
| Rmeas | 0.180 | 0.977 |
| Rpim | 0.057 | 0.301 |
| Number of reflections | 19950 | 2203 |
| <I/σ(I)> | 9.1 | 2.7 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.1 M Bis-Tris pH 5.5, 0.2 M Sodium chloride, and 25% polyethylene glycol 3350 |
