9UMF
Human poly ADP-ribose polymerase(PARP-1 )zinc finger 1 bound to 17bp DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97861 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.393, 36.392, 307.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.950 - 3.500 |
| R-factor | 0.18294 |
| Rwork | 0.180 |
| R-free | 0.23962 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.950 | 3.830 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 4688 | 1059 |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 96.8 | |
| Redundancy | 1.7 | |
| CC(1/2) | 0.975 | 0.955 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 100mM Tris-HCl pH 8.5, 200mM NaCl, 25% PEG3350 |
