9UMD
autoinhibited kpNLRL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2025-03-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97861 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.243, 150.856, 381.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 140.300 - 4.140 |
| R-factor | 0.2564 |
| Rwork | 0.252 |
| R-free | 0.33530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.971 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 140.380 | 4.290 |
| High resolution limit [Å] | 4.140 | 4.140 |
| Rmerge | 0.031 | 0.213 |
| Number of reflections | 34756 | 3414 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.4 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 100 mM HEPES (pH 7.6) 10% PEG3350 |
