9ULP

Crystal structure of FtsB from Streptococcus pyogenes in complex with Nb1 nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	38.364, 60.464, 318.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.180 - 2.550
R-factor	0.25573
Rwork	0.253
R-free	0.30659
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.137
Data reduction software	XDS
Data scaling software	Aimless (0.7.15)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	2.660
High resolution limit [Å]	2.550	2.550
Rmerge	0.115	0.609
Rpim	0.058	0.272
Number of reflections	24247	2703
<I/σ(I)>	10.2	2.7
Completeness [%]	96.0
Redundancy	5.6
CC(1/2)	0.995	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	2% Tacsimate pH 6.0; 100 mM BIS-TRIS pH 6.5, 20% PEG 3350