9ULN

Mogamulizumab Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	110.022, 110.022, 120.971
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.520 - 2.940
R-factor	0.2258
Rwork	0.223
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.292
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.940	2.940
Number of reflections	18517	925
<I/σ(I)>	22.7
Completeness [%]	99.8
Redundancy	9.6
CC(1/2)	0.983	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.02M DL-Glutamic acid H2O, 0.02M DL-Alanine, 0.02M Glycine, 0.02M DL-Lysine monohydrochloride, 0.02M DL-Serine, 0.1M Sodium HEPES, MOPS(acid) pH 7.5, 12.5% v/v MPD, 12.5% PEG1000, 12.5% (W/V) PEG3350