9UL8

Cryogenic temperature crystal structure of Nmar_1308 protein at 2.46 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 31 1 2
Unit cell lengths	75.970, 75.970, 209.420
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.990 - 2.460
R-factor	0.3806
Rwork	0.378
R-free	0.40680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.904
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.990	2.610
High resolution limit [Å]	2.460	2.460
Number of reflections	25601	25543
<I/σ(I)>	10.17
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.990	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		298	0.3M Magnesium chloride hexahydrate, 0.3M Calcium chloride dihydrate, 1.0M Tris (base), BICINE pH 8.5, 40% v/v PEG 500* MME, 20% w/v PEG 2000