9UL7

Improved thermostability of a GH3 glycosidase based on its X-ray crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	112.866, 104.617, 69.156
Unit cell angles	90.00, 91.91, 90.00

Refinement procedure

Resolution	34.559 - 2.200
R-factor	0.1917
Rwork	0.190
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.920
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	273645	40984
<I/σ(I)>	25.643
Completeness [%]	99.9
Redundancy	6.7
CC(1/2)	0.996	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	0.1 M MES pH 6.5 25% (v/v) PEG 550 MME