9UK7
Crystal structure of WDR5 in complex with peptide Ac-MRTEP-NH2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.077, 39.031, 114.962 |
| Unit cell angles | 90.00, 118.89, 90.00 |
Refinement procedure
| Resolution | 38.370 - 1.950 |
| R-factor | 0.1925 |
| Rwork | 0.191 |
| R-free | 0.23710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.027 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.760 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 66726 | 9669 |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.5 | |
| CC(1/2) | 0.985 | 0.784 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v Polyethylene glycol 4,000 |
