9UK1

Crystal structure of WDR5 in complex with EMBOW

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-06
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	P 2 21 21
Unit cell lengths	46.004, 81.043, 108.538
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.000 - 2.020
R-factor	0.1931
Rwork	0.191
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.928
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.940	2.130
High resolution limit [Å]	2.020	2.020
Number of reflections	27509	3922
<I/σ(I)>	9.9
Completeness [%]	100.0
Redundancy	9.3
CC(1/2)	0.995	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M Sodium tartrate dibasic dihydrate, 20% w/v Polyethylene glycol 3,350, pH7.3