9UGI

Room temperature structure of lysozyme by serial synchrotron crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.5
Detector technology	PIXEL
Collection date	2019-02-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	79.040, 79.040, 38.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.520 - 1.800
R-factor	0.1755
Rwork	0.174
R-free	0.20150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.831
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	MOLREP
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	11714	1121
<I/σ(I)>	6.22
Completeness [%]	100.0
Redundancy	46.1
CC(1/2)	0.981	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293.5	sodium acetate, NaCl, PEG 8000