9UEI
Crystal structure of a PhGs rhamnosyltransferase UGT79G15 from Rehmannia glutinosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.745, 71.970, 133.890 |
| Unit cell angles | 90.00, 97.52, 90.00 |
Refinement procedure
| Resolution | 44.370 - 1.880 |
| R-factor | 0.1919 |
| Rwork | 0.190 |
| R-free | 0.22390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.922 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.370 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.107 | 0.041 |
| Number of reflections | 75916 | 2486 |
| <I/σ(I)> | 2.28 | |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 4.5 | 4.6 |
| CC(1/2) | 0.996 | 0.765 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 0. 4 M NaNO3,30% PEG 3350 |
