9UE5

Rhizobium tRNA methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	60.030, 98.449, 78.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.220 - 1.340
R-factor	0.1844
Rwork	0.183
R-free	0.20470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.986
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.220	1.410
High resolution limit [Å]	1.340	1.340
Number of reflections	43559	51861
<I/σ(I)>	18.3
Completeness [%]	83.0	98
Redundancy	3.74
CC(1/2)	0.999	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.1	283.15	20% PEG 3350, 0.2M Ammonium citrate dibasic