9UDZ

Crystal structure of recombinant mushroom Agaricus bisporus mannose-binding protein with a longer C-terminal region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	102.692, 44.121, 77.503
Unit cell angles	90.00, 128.34, 90.00

Refinement procedure

Resolution	40.310 - 2.340
R-factor	0.19088
Rwork	0.187
R-free	0.25872
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eha
RMSD bond length	0.009
RMSD bond angle	1.596
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.310	2.420
High resolution limit [Å]	2.340	2.340
Rmerge	0.133	0.829
Rmeas	0.144	0.895
Rpim	0.054	0.335
Total number of observations		7729
Number of reflections	11521	1106
<I/σ(I)>	11.8	2.2
Completeness [%]	98.8
Redundancy	6.8	7
CC(1/2)	0.997	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	293	sodium phosphate monohydrate, potassium phosphate dibasic