9UCW

anthranilate Phosphoribosyl transferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	113
Detector technology	PIXEL
Collection date	2023-12-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.976
Spacegroup name	P 1 21 1
Unit cell lengths	60.990, 82.660, 73.870
Unit cell angles	90.00, 112.74, 90.00

Refinement procedure

Resolution	28.120 - 2.160
R-factor	0.2168
Rwork	0.214
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ITasser
RMSD bond length	0.009
RMSD bond angle	1.037
Data reduction software	Adxv
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.220
High resolution limit [Å]	2.160	2.160
Number of reflections	69466	2928
<I/σ(I)>	13.9
Completeness [%]	98.1
Redundancy	6.7
CC(1/2)	0.998	0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG8000, Tris, NaCl