9UCE

Crystal structure of glycosyltransferase UGT73K1 in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	P 43 21 2
Unit cell lengths	91.500, 91.500, 167.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.120 - 2.200
R-factor	0.1846
Rwork	0.183
R-free	0.20770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.586
Data reduction software	XDS (Jun 30, 2024)
Data scaling software	XDS (Jun 30, 2024)
Phasing software	PHENIX (1.20.1-4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.200	2.200
Rmeas		0.753
Number of reflections	36792	5809
<I/σ(I)>	15.51	3.95
Completeness [%]	100.0	99.9
Redundancy	24.67	24.41
CC(1/2)	0.998	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium citrate, K/Na tartrate, ammonium sulfate