9UB6

Crystal structure of SFTSV Gn head with nanobody VHH261

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97923
Spacegroup name	P 62 2 2
Unit cell lengths	172.710, 172.710, 136.030
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.200 - 2.890
R-factor	0.1933
Rwork	0.191
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.122
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.200	2.970
High resolution limit [Å]	2.890	2.890
Number of reflections	27305	10
<I/σ(I)>	16
Completeness [%]	99.9
Redundancy	36.7
CC(1/2)	0.998	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Ammonium sulfate, 0.3M Sodium formate, 0.1M Sodium acetate, pH5.0, 3% w/v gama-PGA (Na+ form, LM), 3% w/v PEG 20000