9UAK

Crystal structure of C terminal regulatory domain of cyanobacterial bicarbonate transporter CmpABCD

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-15
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97901
Spacegroup name	C 1 2 1
Unit cell lengths	91.548, 48.935, 96.287
Unit cell angles	90.00, 116.96, 90.00

Refinement procedure

Resolution	27.020 - 1.600
R-factor	0.2035
Rwork	0.202
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.050
Data reduction software	HKL-3000
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.760	1.650
High resolution limit [Å]	1.560	1.560
Number of reflections	52584	7804
<I/σ(I)>	6.6
Completeness [%]	97.4
Redundancy	5.4
CC(1/2)	0.996	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.2	15% PEG 2000 MME,0.1 M KCl,0.1M Tris pH 8.0