9UA8
structure of PTP-MEG2 and IFG1R-pY1165/pY1166 peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2012-12-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.294, 84.707, 52.675 |
| Unit cell angles | 86.03, 94.16, 90.05 |
Refinement procedure
| Resolution | 36.770 - 1.999 |
| R-factor | 0.2186 |
| Rwork | 0.217 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.000 | 1.680 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.070 |
| Number of reflections | 79491 | 32748 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 95.7 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 318 | 20% PEG 4000, 0.2M KSCN, 10% ethelene glycol, 0.1M bis-tris propane |
