9U9Q

Tubulin-RB3-TTL in complex with C38

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	104.000, 159.420, 171.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.380 - 2.930
R-factor	0.2165
Rwork	0.214
R-free	0.26930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.010
Data reduction software	HKL-3000
Data scaling software	xia2
Phasing software	HKL-3000
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.380	3.010
High resolution limit [Å]	2.930	2.930
Number of reflections	50153	4031
<I/σ(I)>	8
Completeness [%]	81.9
Redundancy	5.5
CC(1/2)	0.994	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	6% PEG 4000, 5% glycerol, 0.1M MES, 30mM CaCl2, 30mM MgCl2, pH 6.7