9U9G

Crystal structure of antitoxin HipB from Pseudomonas fluorescens 2P24

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 41
Unit cell lengths	96.630, 96.630, 34.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.160 - 2.150
R-factor	0.2113
Rwork	0.208
R-free	0.27150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.118
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.160	2.227
High resolution limit [Å]	2.150	2.150
Rmerge	0.158	1.431
Number of reflections	17855	1756
<I/σ(I)>	11.1
Completeness [%]	99.8
Redundancy	10.17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Sodium citrate 5.5, and 10 % w/v polyethylene glycol (PEG) 4000