9U8G

Crystal structure of TMPRSS2 in complex with nanobody77_10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 1
Unit cell lengths	67.616, 69.827, 82.192
Unit cell angles	104.97, 112.85, 102.10

Refinement procedure

Resolution	18.290 - 2.000
R-factor	0.1949
Rwork	0.193
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.719
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	18.290	18.290	2.120
High resolution limit [Å]	2.000	5.730	2.000
Number of reflections	81800	3308	12927
<I/σ(I)>	13.18	38.3	1.67
Completeness [%]	96.2
Redundancy	3.18
CC(1/2)	0.998	0.999	0.555

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	100 mM HEPES/ Sodium hydroxide pH 7.0, 10% w/v PEG 6000