9U8E
Crystal structure of Leminorella grimontii GatC in the presence of D-xylose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 87.760, 100.610, 122.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.530 - 3.300 |
| R-factor | 0.2278 |
| Rwork | 0.222 |
| R-free | 0.27860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.521 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.530 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 16782 | 3396 |
| <I/σ(I)> | 4.98 | |
| Completeness [%] | 99.0 | |
| Redundancy | 93.4 | |
| CC(1/2) | 0.934 | 0.413 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8 | 293.15 | 100mM Na-acetate, 100mM Tris-HCl (pH8.0), 40-45% PEG 200, 10mM D-xylose |
