9U7V
The X-ray Crystallographic Structure of oligo-1,6-glucosidase from Paenibacillus sp. STB16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-12-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 154.570, 154.570, 115.174 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.440 - 3.240 |
| R-factor | 0.30715 |
| Rwork | 0.305 |
| R-free | 0.34966 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.919 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.440 | 3.500 |
| High resolution limit [Å] | 3.240 | 3.240 |
| Rmerge | 0.177 | 0.191 |
| Number of reflections | 19869 | 83744 |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 88.1 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293.15 | 0.2M Ammonium acetate, 0.1M Tris pH 8.0, 16% PEG 10000 |
