9U7U

5hmC specific restriction endonuclease Escherichia coli APEC O1 PD-T4-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 64 2 2
Unit cell lengths	123.332, 123.332, 82.932
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.655 - 2.194
R-factor	0.2236
Rwork	0.222
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.834
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.655	2.330
High resolution limit [Å]	2.194	2.194
Rmeas	0.101
Number of reflections	19546	3087
<I/σ(I)>	33.73
Completeness [%]	99.9	99.5
Redundancy	35.23
CC(1/2)	1.000	0.668

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.9	293.15	0.49 M Sodium phosphate monobasic monohydrate, 0.91 M Potassium phosphate dibasic pH 6.9