9U7Q
Chitinase ChiA mutant - C61A/C79A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.258, 50.157, 90.997 |
| Unit cell angles | 90.00, 95.03, 90.00 |
Refinement procedure
| Resolution | 44.457 - 1.600 |
| Rwork | 0.173 |
| R-free | 0.19990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.775 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.460 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.063 | 0.769 |
| Number of reflections | 53051 | 2573 |
| <I/σ(I)> | 17.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.999 | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M Bis-Tris Propane pH 8.5, 0.2M Potassium sodium tartrate tetrahydrate, 20% PEG3350, 10% Ethylene glycol |
