9U77

Crystal structure of Glycogen branching enzyme (VvGBE) from Vibrio vulnificus MO6-24/O

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-17
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	166.800, 106.063, 141.126
Unit cell angles	90.00, 123.30, 90.00

Refinement procedure

Resolution	49.310 - 2.500
R-factor	0.2169
Rwork	0.216
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.602
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmeas	0.211
Number of reflections	70034	3467
<I/σ(I)>	24.1
Completeness [%]	99.4	98.9
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	25% PEG 3350, 0.1M BIS-TRIS pH 6.5, 0.2M Ammonium sulfate