9U75

5hmC specific restriction endonuclease Escherichia coli E. coli O157:H7 PD-T4-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 41
Unit cell lengths	103.629, 103.629, 125.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.911 - 2.803
R-factor	0.2284
Rwork	0.226
R-free	0.28580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.664
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	32637	2158
<I/σ(I)>	21.2
Completeness [%]	100.0	100
Redundancy	13.8
CC(1/2)	0.991	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293.15	0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4,000