9U5V
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q89E variant (complex with LC)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17UM |
| Synchrotron site | SSRF |
| Beamline | BL17UM |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-09 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 53.749, 107.577, 38.932 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.099 - 1.663 |
| Rwork | 0.189 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rhf |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.771 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.099 | 1.718 |
| High resolution limit [Å] | 1.663 | 1.663 |
| Number of reflections | 19963 | 910 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 92.7 | |
| Redundancy | 7.17 | |
| CC(1/2) | 0.996 | 0.581 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 0.2 M Trimethylamine N-oxide dihydrate, 0.1 M Tris pH 8.5, 20% w/v Polyethylene glycol monomethyl ether 2,000 |
