9U4X
Crystal Structure of Alcohol Dehydrogenase from Anoxybacillus geothermalis D9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 293.15 |
| Detector technology | PIXEL |
| Collection date | 2024-02-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.607, 130.769, 134.918 |
| Unit cell angles | 90.00, 90.70, 90.00 |
Refinement procedure
| Resolution | 48.970 - 2.700 |
| Rwork | 0.200 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.433 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.88)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.970 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.082 |
| Number of reflections | 79921 |
| <I/σ(I)> | 7.9 |
| Completeness [%] | 99.8 |
| Redundancy | 2 |
| CC(1/2) | 0.983 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2M Magnesium chloride hexahydrate, 0.1M Bis Tris 5.5, 25% w/v PEG 3350 |
