9U3W

Crystal structure of the ribokinase RBK1 in complex with ribose from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978797
Spacegroup name	C 2 2 21
Unit cell lengths	80.840, 169.060, 117.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.470 - 2.910
R-factor	0.2426
Rwork	0.240
R-free	0.29350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.864
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.530	2.990
High resolution limit [Å]	2.910	2.910
Number of reflections	17881	1303
<I/σ(I)>	14.3
Completeness [%]	99.2
Redundancy	12.3
CC(1/2)	0.997	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.2M Sodium formate, 0.1M Bicine pH 8.6, 24% PEG 5000, 0.04% 1,2-Diaminocyclohexane sulfate, 0.04% Diloxanide furoate, 0.04% Fumaric acid, 0.04% Spermine, 0.04% Sulfaguanidine, 0.004M HEPES sodium pH 6.8