9U39
Crystal structure of Serine Acetyltransferase (SAT) from Planctomyces limnophilus in complex with its substrate serine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-11-24 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54179 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 104.980, 90.030, 122.544 |
| Unit cell angles | 90.00, 114.80, 90.00 |
Refinement procedure
| Resolution | 40.858 - 2.218 |
| Rwork | 0.213 |
| R-free | 0.26920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.892 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.100)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.858 | 2.260 |
| High resolution limit [Å] | 2.218 | 2.220 |
| Rmerge | 0.065 | 0.434 |
| Rmeas | 0.074 | 0.507 |
| Rpim | 0.035 | |
| Number of reflections | 47993 | 2225 |
| <I/σ(I)> | 11.94 | |
| Completeness [%] | 93.3 | 78.57 |
| Redundancy | 4.2 | 3.4 |
| CC(1/2) | 0.999 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | 35% v/v 2-methyl-2,4-pentanediol, 100mM HEPES pH 7.5, 200mm NaCl |
