9U0G
Crystal structure of the apo BRL2 ectodomain from Arabidopsis thaliana (monoclinic crystal form).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-12-05 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.900, 211.610, 67.170 |
| Unit cell angles | 90.00, 112.61, 90.00 |
Refinement procedure
| Resolution | 63.910 - 2.600 |
| R-factor | 0.232 |
| Rwork | 0.231 |
| R-free | 0.25910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.493 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.910 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmeas | 0.011 | 0.705 |
| Number of reflections | 118705 | 8604 |
| <I/σ(I)> | 7.6 | 1.4 |
| Completeness [%] | 99.4 | 97.4 |
| Redundancy | 3.6 | 3.4 |
| CC(1/2) | 0.996 | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 22% PEG 3,350, 0.2 M Na citrate, 0.1 M Bis-Tris pH 7.0 |
