9U00
Crystal structure of NbH10, a nanobody against the PWWP domains of LEDGF and HRP-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-01 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96779 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.864, 53.864, 348.524 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.090 - 2.320 |
| R-factor | 0.2719 |
| Rwork | 0.269 |
| R-free | 0.32170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.537 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.1_5286: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.090 | 2.642 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.307 | 3.624 |
| Rmeas | 0.312 | 3.680 |
| Rpim | 0.052 | 0.616 |
| Total number of observations | 15652 | |
| Number of reflections | 9033 | 452 |
| <I/σ(I)> | 12.2 | 1.7 |
| Completeness [%] | 63.2 | |
| Redundancy | 36.5 | 34.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1 M Succinic acid 1% PEG MME 2000 0.1 M HEPES pH 7.0 |
